We are excited to share a set of milestones from our collaboration with Revvity that make SemaCyte multiplexing even easier to adopt across high content imaging teams.

A new SemaCyte analysis building block in Harmony and Signals Image Artist

Revvity has released a SemaCyte Analysis Building Block that supports SemaCyte detection and optical barcode deconvolution inside Harmony and Signals Image Artist.

• Harmony integration is a major win for Opera Phenix and Opera Phenix Plus users, enabling a familiar, end to end workflow from image acquisition through barcode driven segmentation and downstream feature extraction.

• Signals Image Artist support extends the same core capability into a platform that can ingest images from multiple microscope systems and is often deployed at scale across large pharma organisations as a shared image analysis environment. That makes it easier for distributed teams and multi site groups to roll out standardised SemaCyte analysis without changing their existing imaging hardware choices.

Published technical content demonstrating practical multiplexing

Revvity has also published an application note describing a complete workflow for cell line multiplexing with SemaCytes, including barcode deconvolution and downstream analysis for high content imaging throughput gains.

This is an important point for adoption: the workflow is not just technically possible, it is operationally straightforward for teams who already work in Harmony or Signals Image Artist and want to add multiplexing with minimal process change.

Link: https://www.revvity.com/gb-en/content/increasing-high-content-imaging-throughput-cell-line-multiplexing

Joint webinar with Fraunhofer to showcase multiplexed, data dense cell painting

To complement the application note, we ran a webinar together with Revvity and Fraunhofer, demonstrating how SemaCytes can be used to multiplex cell models for accelerated, data dense cell painting, and how the analysis can be executed cleanly within established high content workflows.

Link: event.on24.com/wcc/r/5106123/3CEBAB34709855DBC6999B2C04EEBAE2?partnerref=hcsEloqua2&utm_campaign=LSC-HCS-2025-GLOBAL-Email-TNA-EGM-REV-ZZ-SemarionFraunhoferWeb&utm_medium=email&utm_source=Eloqua&sfdc_id=701Pp00001RNIdhIAH&LS=Email-Outbound

Revvity Image of the Month featuring SemaCytes

Revvity also highlighted SemaCytes in their Image of the Month series, showcasing the visual quality and consistency you can achieve when running imaging assays on SemaCyte microcarriers.

Link: https://x.com/RevvityInc/status/1990406317706547557

Why this matters

SemaCytes unlock a simple idea with big consequences: pool multiple adherent cell models in the same well, then computationally deconvolute them using optical barcodes. That can increase throughput, reduce plate count and reagent usage, and make richer experimental designs practical.

Our collaboration with Revvity makes that value easier to realise for organisations already invested in Revvity’s ecosystem:

• Harmony for rapid adoption by Opera Phenix users

• Signals Image Artist for scalable deployment across large organisations and heterogeneous imaging fleets

• While still preserving the flexibility to run SemaCyte assays and analysis through Semarion’s own software workflows where that is preferred