The SemaPlex Multiplexing Platform
With the SemaPlex Multiplex Platform, it is possible to optically barcode adherent cells for high-order multiplexing and pooling. Millions of unique identifiers can be encoded in the walls of SemacCyte microcarriers. These barcodes are viewed with brightfield microscopy and digitally deconvoluted with the Semalyse software.
Multiplexing and combining various adherent cell types into one well enables pooled cell screening which accelerates discovery campaigns and reduces the resource burden of cell assaying.
Barcoding Adherent Cells
Multiplex cell models to perform pooled assays
2-10x less time and cost
for cell panel screening
e.g. rapid imaging of multiple cell models drugged within the same well
Intrawell positive & negative controls for imaging
and quality of data
e.g. add normalisation controls into
every individual well
Multi-cell cultures to create deconstructed organ models
create complex co-culture
systems with 2-10 cells
e.g. organotypic models with spatially separated and barcoded cells
Multiplexing Adherent Cells for Pool Cell Assays
Different cell types are seeded on a different SemaPlex array, each with a unique optical barcode at the edge of the cell growth area.
After the cells have attached and the desired morphology is achieved, microcarriers with barcoded cells can be collected and cryopreserved.
Vials of microcarriers with different barcodes and cell types can be pooled together and dispensed into a microplate for assaying.
After assaying, the Semalyse software identifies the microcarriers, deconvolutes their barcodes, and digitally tiles them together for further analysis.