The SemaPlex Multiplexing Platform

With the SemaPlex Multiplex Platform, it is possible to optically barcode adherent cells for high-order multiplexing and pooling. Millions of unique identifiers can be encoded in the walls of SemacCyte microcarriers. These barcodes are viewed with brightfield microscopy and digitally deconvoluted with the Semalyse software.

Multiplexing and combining various adherent cell types into one well enables pooled cell screening which accelerates discovery campaigns and reduces the resource burden of cell assaying.

Barcoding Adherent Cells

Multiplex cell models to perform pooled assays

2-10x less time and cost
for cell panel screening

e.g. rapid imaging of multiple cell models drugged within the same well

Intrawell positive & negative controls for imaging

improving reproducibility
and quality of data

e.g. add normalisation controls into
every individual well

Multi-cell cultures to create deconstructed organ models

create complex co-culture
systems with 2-10 cells

e.g. organotypic models with spatially separated and barcoded cells
Access to our mutliplexing solutions is possible through our partnership programmes.

Multiplexing Adherent Cells for Pool Cell Assays

Different cell types are seeded on a different SemaPlex array, each with a unique optical barcode at the edge of the cell growth area.

After the cells have attached and the desired morphology is achieved,  microcarriers with barcoded cells can be collected and cryopreserved.

Vials of microcarriers with different barcodes and cell types can be pooled together and dispensed into a microplate for assaying.

After assaying, the Semalyse software identifies the microcarriers, deconvolutes their barcodes, and digitally tiles them together for further analysis.

Resources

SemaCyte® Microcarriers Platform - Brochure

Platform
The SemaCyte® Microcarrier Platform leverages novel materials physics to move and freeze adherent cells. Our approach introduces flexibility, speed, and into existing drug discovery workflows.
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SemaCyte® Microcarriers for Flexible Imaging Assays

Applications
SemaCytes increase the workflow flexinility during drug screening assays with live or fixed cell imaging.
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SemaCyte® Microcarriers for Multi-Day Drug Sensitivity Assays

Applications
Assay-ready A549 seeding shuttles for multi-day drug sensitivity assays against Nutlin-3A and Trametinib
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