The SemaCyte® Multiplexing Platform

With the SemaCyte® Multiplex Platform, it is possible to optically barcode adherent cells for high-order multiplexing and pooling. Millions of unique identifiers can be encoded in the walls of SemacCyte microcarriers. These barcodes are viewed with brightfield microscopy and digitally deconvoluted with the Semalyse software.

Multiplexing and combining various adherent cell types into one well enables pooled cell screening which accelerates discovery campaigns and reduces the resource burden of cell assaying.

Barcoding Adherent Cells

Multiplex cell models to perform pooled assays

2-10x less time and cost
for cell panel screening

e.g. rapid imaging of multiple cell models drugged within the same well

Intrawell positive & negative controls for imaging

improving reproducibility
and quality of data

e.g. add normalisation controls into
every individual well

Multi-cell cultures to create deconstructed organ models

create complex co-culture
systems with 2-10 cells

e.g. organotypic models with spatially separated and barcoded cells
Access to our SemaCyte® Multiplexing solution is available through our Early Adopter Programme.

Multiplexing Adherent Cells for Pool Cell Assays


Cells are simply seeded onto arrays of immobilized carriers on cell culture plasticware. After collection, these carriers can be cryopreserved or pooled and dispensed into microplates, streamlining assay workflows.

Broad Compatibility

SemaCyte microcarriers harmonize with various data analytics tools, assay types, read-out equipment, and lab automation tools, ensuring widespread adoption across life sciences.

For more information of the products, protocols, and experimental examples click here.

Different cell types are seeded on SemaCyte arrays, each with a unique optical barcode at the edge of the cell growth area.

After the cells have attached and the desired morphology is achieved,  microcarriers with barcoded cells can be collected and cryopreserved.

Vials of microcarriers with different barcodes and cell types can be pooled together and dispensed into a microplate for assaying.

After assaying, the Semalyse software identifies the microcarriers, deconvolutes their barcodes, and digitally tiles them together for further analysis.

Product Protocol – SemaCyte® Seeding Dish 20 Multiplex (SD20-MX)

Protocol to generate adherent assay-ready cells on SemaCyte® microcarriers, pool barcoded cells, and perform multi-day drug assays with immunostaining endpoints.
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The SemaCyte® Multiplexing Platform: Accelerating Image-Based Cell Panel Screening

The SemaCyte Multiplexing Platform pools diverse cell types within a single well, for a 6-fold reduction in screening time and 10-fold reduction in cost. This technology note outlines the platform, the workflow, the data analytics, and its potential.
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The SemaCyte® Multiplex Platform: Unravelling Nutlin-3a Cytotoxicity and p53 Induction

In this study, we employ a 4-plex cell panel as a proof of concept. Our results demonstrate that the SemaCyte® Multiplex platform establishes meaningful and biologically plausible associations between Nutlin-3a cytotoxicity and p53 induction. Specifically, we investigate A549, PC3, T98G, and HepG2 cells, which were pooled and exposed to serial dilutions of Nutlin-3a.
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